Comprehensive sexuality education (CSE) has gained global recognition as a vital effort to empower adolescents and young people; enable them to improve and protect their health, well-being and dignity; and support them in developing critical thinking skills, citizenship, and equal, healthy and positive relationships. This toolkit offers guidance and resources on ways to deliver CSE for children, adolescents, and youth in non-formal and formal settings and encourage other stakeholders to develop and implement CSE policies and programmes.