This Gender Equality Assessment of Provincial Strategic Plans and Provincial Operational Plans on HIV, STIs and TB within South Africa assesses provincial HIV, STI and TB responses against the HEARD and ATHENA Framework for Women, Girls and Gender Equality and the UNAIDS Gender Assessment Tool for National HIV Responses. The Assessment identifies the extent to which provincial responses prioritise women, girls and gender equality by including a number of specific, evidence-informed interventions; it furthermore highlights policy and programmatic gaps and challenges within these plans. It concludes by identifying opportunities for strengthening HIV, STI and TB responses for women, girls and gender equality at provincial and local level.